11/16/2023 0 Comments Serial dilution![]() ![]() Transfer 5cm 3 of this solution into a test tube. Measure out 10cm 3 of the 2M glucose solution into an empty test tube. Medical and pharmaceutical personnel are constantly dealing with dosages that require concentration measurements and dilutions. The amount of distilled water will depend what dilution factor you are aiming for, but in this example we will use 5cm 3 of water because we are going to dilute by a factor of two each time, making the total volume to 10cm 3. One of the most common series doubles the dilution factor with each transfer (1:2, 1:4, 1:8. A small amount of serum or solute can be serially diluted by transferring aliquots to diluent. ![]() Of course, the addition of the stock solution affects the total volume of the diluted solution, but the final concentration is likely close enough even for medical purposes. Serial dilution is a common technique used in many immunologic procedures. If the stock solution is 10.0% KCl and the final volume and concentration need to be 100 mL and 0.50%, respectively, then it is easy to calculate how much stock solution to use: For example, if you add a 1 mL sample to 9 mL of diluent to get 10 mL of solution, DF V i V f 1mL 10mL 1 10. This new solution will have your desired concentration (C 2 ). Then, add enough diluting liquid (water, etc.) to make a total volume V 2. The dilution factor or the dilution is the initial volume divided by the final volume. Serial dilution is a commonly used technique that generates a low-concentration working sample from a high-concentration stock solution and is used to set up screening conditions over a large dynamic range for biological study, optimization of reaction conditions, drug screening, etc. When you know all four values in the equation C 1 V 1 C 2 V 2, perform your dilution as follows: Measure the volume V 1 of the solution with concentration C 1. Medical personnel commonly must perform dilutions for IV solutions. In serial dilutions, you multiply the dilution factors for each step. ![]()
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